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. 2022 Nov 24;14(12):2594. doi: 10.3390/pharmaceutics14122594

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression levels of necroptosis-related proteins of HT1080 cells exposed to free doxorubicin (DOX) and DOX-loaded MCP (MCP-D) and DOX-loaded MSP (MSP-D) at an equivalent DOX concentration of 1 μg/mL for 4 h with and without UV light irradiation for 10 min (A). Cell lysates were extracted and protein expression was detected using Western blot. GAPDH was used as internal control for equal loading. Quantitative evaluation of MLKL and PIP-1 proteins expression was relative to control group (B) (* p < 0.05, ** p < 0.005).