Table 1.

Comparison of platforms developed for the manufacturing CAR-T cell therapies.

Name	Company	Principle	Volume/Surface Area	Scalability	Gene Editing?	Temperature Control	Final formulation
CliniMACS Prodigy	Miltenyi Biotec	CentriCultUnit or External culture vessel	low/dependant on external vessel size	low	In place	Requires external temperature control when external vessel used. No reagent temperature control	Fill and finish capable
Cocoon	Lonza	Customizable cassette	460 mL—low	medium	In place	Duel environmental control for reagents and cell growth	Fill and finish capable
Xuri cell	Cytiva	Wavebag	0.3–25 L—medium	medium	In place	Integrated tray heater and sensors	External finishing required
Duet Pump	FibreCellSystems	Hollow fibre membrane	80 cm2–1.2 m2	medium	No	Requires CO2 Incubator	External finishing required
Quantum	TERUMO	Hollow fibre membrane	1.7–2.1 m2	High	No	Continuous control of temperature	External finishing required
HF Primer	CellCultureCompany	Hollow fibre membrane	1.5 m2	medium	No	Requires CO2 Incubator	Concentrates harvest but external finishing required
AutovaxID	CellCultureCompany	Hollow fibre membrane	80–100 L equivalent	High	No	Automated control of temperature	Integrated refrigerator for continuous harvest
AcuSyst-Maximizer	CellCultureCompany	Hollow fibre membrane	80–200 L equivalent	High	No	Automated control of temperature	Integrated refrigerator for continuous harvest and In-line filter for harvest clarification to reduce downstream processing
AcuSyst-Xcellerator	CellCultureCompany	Hollow fibre membrane	500–2000 L equivalent	High	No	Automated control of temperature	Integrated refrigerator for continuous harvest and In-line filter for harvest clarification to reduce downstream processing