Figure 1.

Establishment of radio-iodine refractory PTC cell lines. (A). MTT tests suggested BCPAP and K1 treated with ¹³¹I doses of more than 30 μCi showed toxicity in two cell lines. (B). RAIU of two cell lines incubated with differing doses of ¹³¹I for 12 h. 125I uptake in two cells lines with 15 μCi ¹³¹I decreased most significantly compared with that of the control. (C). RAIU of two cell lines incubated with 15 μCi ¹³¹I for different time periods. Compared with the control, ¹²⁵I uptake of every experimental group gradually decreased. (D). RAIU were significantly decreased in treated BCPAP and K1 compared with that of the control. (E). The expression of the thyroid-specific protein (NIS, TPO, TSHR) were significant decreased in treated BCPAP and K1 compared with that of the control. * p < 0.05, ** p < 0.01, **** p < 0.0001.