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. 2022 Dec 7;12(12):2017. doi: 10.3390/jpm12122017

Figure 3.

Figure 3

NIS is a direct target of miR-1296-5p. (A). The miR-1296-5p was abundantly detected in RAIR PTC tissue and cells. (B). Predicted binding site in the 3′-UTRs of NIS. The red flame is the representative image. scale bare, 20 μm (C). Dual luciferase reporter assays in 293T cells. (D). The basal expression levels of miR-1296-5p in K1 and BCPAP cells. (E). MiR-1296-5p expression was significantly upregulated after transfection with miR-mimic but downregulated after transfection with miR-inhibitor. (F,G). qRT-PCR and Western blotting showed that miR-1296-5p could inhibit NIS expression both in mRNA and protein levels. (H). schematic of the model. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.