Table 7.
Characterization of carbapenemases into MBL and KPC on phenotypic basis and detection of carbapenemases encoding genes (n = 250).
| Isolates | Phenotypic Methods | Total (%) | Manual PCR | Microarray Assay | ||||
|---|---|---|---|---|---|---|---|---|
| bla NDM | bla KPC | Unrecognized | bla NDM | bla KPC | Unrecognized | |||
|
E. coli (n = 142) |
MBL | 107 (75.35%) | 109 (76.7%) |
24 (16.90%) |
9 (6.33%) |
111 (78.16%) | 24 (16.90%) |
7 (4.92%) |
| KPC | 21 (14.78%) |
|||||||
| Unrecognized | 14 (8.85%) | |||||||
|
K. pneumoniae (n = 91) |
MBL | 61 (67.03%) | 64 (70.3%) |
27 (29.67%) |
00 | 64 (70.3%) |
27 (29.67%) |
0 |
| KPC | 27 (29.67%) | |||||||
| Unrecognized | 3 (3.29%) |
|||||||
|
E. cloacae (n = 7) |
MBL | 5 (71.42%) | 5 (71.42%) | 2 (28.58%) | 00 | 5 (71.42%) | 2 (28.58%) | 0 |
| KPC | 2 (28.58%) | |||||||
| Unrecognized | - | |||||||
|
P. mirabilis (n = 10) |
MBL | 5 (50.0%) | 5 (50.0%) | 5 (50%) | 2 (20%) | 5 (50%) | 5 (50%) | 0 |
| KPC | 3 (30.0%) | |||||||
| Unrecognized | 2 (20%) | |||||||
| Total (n = 250) | MBL | 178 (71.2%) | 183 (73.2%) | 55 (22%) | 11 (4.4%) | 188 (75.2%) | 55 (22%) | 7 (2.8%) |
| KPC | 53 (21.2%) | |||||||
| Unrecognized | 19 (7.6%) | |||||||