Figure 1.
Dynamics of emergence and replication activities of 5′ terminally deleted CVB RNA forms in the pancreas. (A) Viral loads were quantified in the pancreas of CVB3/28-infected DBA/2J mice by classic RT-qPCR assay (n = 4 to 6, each dot represents one mouse). The dotted line represents the significance threshold of 103 genome copy (gc)/microgram of total nucleic acid extracted. (B) Viral titers were measured in the pancreases of CVB3/28-infected DBA/2J mice by classic plaque-forming unit assay (PFU) (n = 5, each dot represents one mouse). (C,D) The respective viral loads of EV-B full-length (FL) and 5′TD were measured using a RACE-PCR method associated with micro-electrophoresis (Agilent®) in the pancreases of CVB3/28-infected mice (n = 4 to 8). (A–D) Data represent mean +/− standard error of the mean (SEM). (E) 5′ TD/FL ratio in the pancreases of CVB3/28 infected mice from 8 HPI to 7 DPI. Multiple comparison test of the 5′ TD/FL ratio. ****: p < 0.0001, multiple comparisons test. (F) Linear regression curves and Spearman R coefficients of correlation between viral loads and 5′ TD EV-B proportions (2 to 7 DPI; n = 15, each dot represents one mouse). DPI: days post infection. HPI: hours post infection. CVB: coxsackievirus. TD: 5′ terminally deleted. FL: full-length. nt: nucleotides.