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. 2022 Dec 13;14(12):2781. doi: 10.3390/v14122781

Figure 4.

Figure 4

Impact of CVB3/28 and CVB-TD RNA populations’ infections on insulin maturation in pancreases of immunocompetent mouse. (A) Insulin (Ins1) mRNA levels’ fold-changes were measured by RT-qPCR in the pancreases of CVB3/28-infected mice at 3 and 28 days post-infection (DPI) (n = 5). (B) Using specific murine proinsulin ELISA kit, proinsulin levels in blood were measured in uninfected mice and CVB3/28-infected mice at 3 and 28 DPI (n = 5). (C) Blood insulin was measured using a specific ELISA kit in uninfected and CVB3/28-infected mice at 3 and 28 DPI (n = 5). (D) PC2 (PCSK2) mRNA levels’ fold-changes were quantified by RT-qPCR in pancreases of CVB3/28-infected mice and of mice inoculated with homogenized pancreases from 3, 14 and 28 DPI (n = 5). (E) Anti-GAD65 and anti-IA2 antibodies levels in the blood of uninfected mice, CVB3/28-infected mice and of mice inoculated with homogenized pancreases from uninfected, 3, 14 and 28 DPI mice, at 3 and 28 DPI (n = 3 to 4) (uninfected mice were sacrificed at 3 or 14 DPI). Data represent mean +/− SEM for three independent experiments. Mann–Whitney U test; *: p < 0.05; **: p < 0.01; ****: p < 0.0001. ns: non-significant. DPI: days post infection. Ins1: preproinsulin gene. PCSK2: Proprotein Convertase Subtilisin/Kexin Type 2. GAD65: glutamic acid decarboxylase 65. IA-2: islet antigen 2.