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. 2022 Dec 7;479(23):2395–2417. doi: 10.1042/BCJ20220417

Figure 7. EBNA2 and EBNA3 proteins attenuate NFAT activity.

Figure 7.

(A) EBV-negative BL cells expressing a conditionally active (ER)-EBNA2 fusion protein (BL41K3) were transfected with 5 μg of empty plasmid (pGL3 basic) or the pGL3_NFAT luciferase reporter and 2.5 μg of control plasmid pRL-TK. Cells were incubated for 24 h in the absence or presence of β-estradiol followed by an 18 h incubation ± anti-IgM and analysed for luciferase activity. Firefly signals were normalised to Renilla luciferase signals from the co-transfected control plasmid pRL-TK. Results show the mean ± standard deviation from two independent experiments analysed in duplicate. (B) EBV-negative BL31 cells or cells infected with wild-type EBV Bacmid (BL31 wt Bac2) or EBNA3A, 3B and 3C knock-out EBV (BL31 EBNA3 KO) were transfected and incubated with anti-IgM as above.