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. 2001 Jan;69(1):315–324. doi: 10.1128/IAI.69.1.315-324.2001

FIG. 3.

FIG. 3

PCR analysis of the left and right junctions. (A) PCR analysis identified the left junction at 60.03 min adjacent to orf360 of the E. coli chromosome in strain E2348/69 and EPEC serotype O142:H6, both members of the EPEC1 category (48). PCR with primer pair 1 (K988 and K1072) resulted in an amplicon of 584 bp using K-12 strain MG1655 DNA as the template, whereas primer pair 2 (K988 and K1071) resulted in an amplicon of 716 bp using E2349/69 DNA as the template. (B) Similarly, the right junction was at 59.35 min within the ssrA gene of the the E. coli chromosome in strain E2348/69 and EPEC serotype O142:H6. PCR with primer pair 3 (J8 and J10) amplified a K-12-specific amplicon of 458 bp, whereas primer pair 4 (J8 and K1062) amplified an E2348/69-specific amplicon of 1,416 bp.