Figure 2.

Imino protons region of the ¹H NMR titration spectra of c-kit21T12T21 with (a) aloe emodin (1) and (b) aloe emodin-8-glucoside (2) (R = 2.0 after two hours of the addition). The numbers indicate the assignation to the corresponding imino protons, the dotted lines highlight the change in chemical shifts. The asterisks (*) mark the presence of new signals between 11.2 and 10.6 ppm. Spectra were acquired at 25 °C in 90% H₂O and 10% D₂O, 20 mM KCl, and 5 mM K-phosphate buffer, pH 6.9.