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. Author manuscript; available in PMC: 2023 Dec 6.
Published in final edited form as: Biochemistry. 2022 Nov 15;61(23):2709–2719. doi: 10.1021/acs.biochem.2c00528

Figure 5.

Figure 5.

Selected regions of 1H-15N TROSY-HSQC (left) and 1H-15N HSQC (right) spectra of full-length 15N(304-393)-p53 free (black) and bound to a 60-bp DNA with random nucleotide overhangs (violet, DNA-L sequence), with (ATG)n repeat overhangs (red) and with p21-native sequence overhangs (gold). Differences in cross-peak lineshapes between the left and right spectra are due to differences in experimental setup (TROSY vs non-TROSY) and different isotopic labeling schemes (2H labeling in TROSY-HSQC, 1H in HSQC). Spectra were recorded in NMR buffer (containing 150 mM NaCl) at 25°C, and all samples used a protein-DNA ratio of 4:1.