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. 2001 Jan;69(1):325–335. doi: 10.1128/IAI.69.1.325-335.2001

FIG. 3.

FIG. 3

In vitro transcription-translation of the linear pFLL97 plasmid carrying the vimA gene. Five microliters of each 50-μl reaction mix was subjected to acetone precipitation and analyzed by SDS-PAGE. The plasmid vector (pT7-5 [61]) and the recombinant plasmid pFLL97 were linearized by ScaI to prevent expression of the amp gene. Molecular masses are shown on the left. Lanes: A, pT7-5; B, pFLL97 carrying the vimA gene. Proteins were labeled with [35S]methionine and visualized by autoradiography.