FIG. 7.

Proteolytic activity of P. gingivalis mutants. P. gingivalis was grown to late log phase (OD₆₀₀ of 1.1) in 500 ml of BHI broth supplemented with hemin and vitamin K. Activity against BAPNA (A) and Z-lysine-dl-p-nitroanilide (B) was tested in whole-cell culture by the method of Potempa et al. (38). The results shown are representative of four independent experiments.