TABLE 1.
E. coli and P. gingivalis strains and plasmids used in this study
| Strain or plasmid | Genotype and phenotype | Reference |
|---|---|---|
| Strains | ||
| E. coli, NM522 | supE thi Δ(lac-proAB) Δ(hsdMS-mcrB)5 (F′ proAB+lacIqlacZΔM15) | 17 |
| P. gingivalis | ||
| W83 | Wild type (black pigmented, proteolytic) | |
| FLL32 | recA::ermF-ermAM (non-black pigmented, nonproteolytic) | 1 |
| FLL33 | recA::ermF-ermAM (black pigmented, proteolytic) | 14 |
| FLL92 | vimA::ermF-ermAM (non-black pigmented, nonproteolytic) | This work |
| FLL92.1 | vimA::ermF-ermAM (non-black pigmented, nonproteolytic) | This work |
| Plasmids | ||
| pT7-5 | Apr T7 φ10 | 61 |
| pFLL97 | pT7-5 carrying the vimA gene | This work |
| pUC19 | AprlacIPOZ′ | 66 |
| pVA2198 | Spr Emr | 15 |
| pFLL90 | Apr, pUC19 carrying 1.6-kb DNA fragment including the intact vimA gene | This work |
| pFLL91 | Apr Emr, pFLL90 with vimA gene interrupted with ermF-ermAM cassette | This work |