TABLE 2.
Ability of IgG antibodies to protect mice in vivo
| Hybridoma | Isotype | Avidity (M−1) | No. surviving/total at antibody dose (μg/mouse) ofa:
|
||||
|---|---|---|---|---|---|---|---|
| 0.3 | 1 | 3 | 10 | 30 | |||
| Hyp6BG5 | IgG2a | 2.35 × 107 | NDb | ND | 0/4 | 0/5 | 0/5 |
| Hyp6BG6 | IgG2b | 3.79 × 108 | ND | ND | 0/4 | 0/5 | 0/5 |
| Hyp6BG7 | IgG1 | 1.86 × 109 | 1/5 | 2/5, 3/5c | 4/4 | ND | ND |
| Hyp6BG8 | IgG1 | 1.51 × 1010 | 1/5 | 5/5, 5/5 | 4/4 | ND | ND |
a
Ratios of the number of mice surviving on day 35 over the total number of mice used for the experiment are shown.
b
ND, not done.
c
Two experiments were performed with Hyp6BG7 and Hyp6BG8 at 1 μg/mouse. In the first experiment, the results were 2 of 5 versus 5 of 5 for Hyp6BG7 and Hyp6BG8. In the second experiment, the results were 3 of 5 versus 5 of 5. By Fisher's exact test, P = 0.17 for 2 of 5 versus 5 of 5 and 0.033 for 5 of 10 versus 10 of 10.