Figure 4.

IL-33 deficiency inhibited Th17 cells in murine ARDS. (a) The percentage of CD4+ IL-17+CD4+ T cells in the lung was detected by flow cytometry. (b) The mRNA expression of RORγt in the lung was measured by real-time PCR. (c) The protein expression of RORγt in lung tissue was measured by Western blotting. (d) The concentrations of IL-17 and IL-22 in BALF were detected by ELISA. The data are expressed as the means ± SDs. Significant differences are shown by ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 by one-way ANOVA followed by Bonferroni's post hoc test comparing the WT control, LPS, LPS+rmIL-33, and LPS+IL-33^−/− groups. N = 3‐5 for each group, and three independent repeated experiments were carried out.