Fig. 2.

Disruption of BnaPPT1 genes affects plant growth of B. napus. (a) Growth phenotype of overexpression lines (OE1 and OE11) and mutants (CR5, CR105, CR28 and CR54). Pictures were taken from two-week-old plants. Bars = 2 cm. (b) Chlorophyll content in leaves. Samples were taken from three-week-old plants. Chla, Chlorophyll a; Chlb, Chlorophyll b; Car, Carotenoid. Values are means ± SD (n = 3). (c) Content of chlorophyll precursors from three-week-old plants. Values are means ± SD (n = 3). Proto-IX, protoporphyrin IX; Mg-Proto-IX, Mg protoporphyrin IX; Pchl, protochlorophyllide. (d) The transverse sections of leaves were observed under transmission electron microscope. Red arrows indicate the thylakoid grana. Samples were taken from leaves of 30-day-old plants. (e)-(h) Chloroplast number per cell, starch granule number per cell, starch granule number per chloroplast and size per starch granule. Values are means ± SD (n = 8–10). (i)-(l) Photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO₂ concentration in leaves. Data were collected from 80-day-old plants. Values are means ± SD (n = 5). Different letters represent significant differences at P < 0.05, based on an ANOVA analysis with Fisher LSD test. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)