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. 2022 Mar 16;42:163–176. doi: 10.1016/j.jare.2022.03.009

Fig. 1.

Fig. 1

Tobacco seed deterioration and screening by controlled deterioration. (A) germination progress curve of K326 tobacco seeds with different controlled deterioration degrees. controlled deterioration condition: adjust the seeds water content to 20% and seal them with aluminum foil compound bags. Each bag contains 1 g seeds. A total of 21 aluminum foil bags. All the bags were placed at 45℃ constant temperature, and three bags (three repetitions of a sample) were taken out every 12 h for germination test. CD0h, CD12h, CD24h, CD36h, CD48h, CD60h, and CD72h respectively represent controlled deterioration treatment for 0 h, 12 h, 24 h, 36 h, 48 h, 60 h and 72 h. (B) Germination energy. (C) Germination percentage. (D) Mean germination time. (E) Vigor index. (F) Germination progress curve of K326 (CK) tobacco seeds with long-term storage (LS) and controlled deterioration for 60 h. (G) Peroxidase (POD) activity; (H) Catalase (CAT) activity; (I) Superoxide dismutase (SOD) activity; (J) Glutathione reductase (GR) activity. LSD (α = 0.01) method was used for multiple comparison.