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. 2022 Mar 16;42:163–176. doi: 10.1016/j.jare.2022.03.009

Fig. 5.

Fig. 5

Validation of rice homologous genes OsARF18 and OsMBD707. (A) The background of Osarf18 and Osmbd707 mutants. Mutants were created in the Oryza sativa Japonica cv. ZH11 background by CRISPR/Cas9 method [31]. (B-C) The rice seedlings after 14 days of a standard germination test, in which ZH11, Osarf18-1, Osarf18-2, Osmbd707-1 and Osmbd707-2 have been stored for 8 months at room temperature. The bar represents 50 mm. (D-F) Standard germination test of ZH11, Osarf18 and Osmbd707 stored for 4 and 8 months. (D) Germination percentage; (E) Germination index; (F) Vigor index. (G) The fold change of germination index and vigor index, which is represented by the percentage of difference between 4 months of storage and 8 months of storage. (H-I) Rice seedlings after 14 days of a standard germination test, in which ZH11, Osarf18-1, Osarf18-2, Osmbd707-1 and Osmbd707-2 have been treated for 72 h of controlled deterioration. The bar represents 50 mm. (J-L) Standard germination test of ZH11, Osarf18 and Osmbd707 before and after 72 h of controlled deterioration. (J) Germination percentage; (K) Germination index; (L) Vigor index. (M) The fold change of germination index and vigor index, which is represented by the percentage of difference before and after 72 h of controlled deterioration. LSD (α = 0.01) method was used for multiple comparison.