FIG. 1.

Adhesion of metacyclic trypomastigote surface glycoprotein gp90 to host cells is inhibited by the recombinant protein containing the C-terminal domain of the molecule. The assay was carried out as described in the text by incubating HeLa cells for 1 h at 37°C with native gp90 at 20 μg/ml in the absence or presence of the indicated concentrations of r-gp90. HeLa cells incubated with gp90 plus GST or J18b, the recombinant protein containing the C-teminal domain of gp82, served as controls. The binding of gp90 was revealed by MAb 1G7, which recognizes the native molecule but not the recombinant protein. Representative results of one of three experiments are shown. Values are the means ± standard deviation (SD) of triplicates.