FIG. 2.

Oligonucleotides and target gp90 gene encoding the T. cruzi metacyclic trypomastigote surface glycoprotein. (A) Nucleotide sequence of gp90 analyzed in this study, aligned with that of gp82 for comparison. The regions on which the oligonucleotides complementary to gp90 were based are indicated (PS2, PS4, PS5, PS6, and PS7). (B) Schematic representation of the currently known portion of the gp90 gene, which corresponds to that encoding the C-terminal domain of the molecule. The arrows represent the various phosphorothioate oligonucleotides (not drawn to scale): PS1, based on a sequence of the gp90 coding strand; PS2, the antisense counterpart of PS1; PS3, with the same base composition as PS2 but mismatched sequence; and PS4 to PS7, complementary to different gp90 sequences.