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. 2022 Dec 23;13:7923. doi: 10.1038/s41467-022-35657-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a SA-β-gal/EdU images and b quantification of non-irradiated (NS) and irradiated senescent (Sen IR) melanocytes. Scale bar = 100 μM. c mRNA analysis of p16, p21, IL6, and MMP1. Expression was normalized using Cq values of tubulin. d mRNA expression of Bcl-2, Bcl-xL, and Bcl-w from irradiated melanocytes. Expression was normalized using Cq values of tubulin. e Protein expression and densitometry analysis of BCL-2, Bcl-xl, and Bcl-w from irradiated melanocytes. Vinculin was used as a housekeeping protein and expression was normalized to protein levels in non-irradiated non-senescent cells. f Viability of non-irradiated and irradiated melanocytes treated with indicated concentrations of ABT-263 for 24 h. g SA-β-gal/EdU images and h quantification of puromycin vector transduced (NS) and BRAF^V600E vector transduced senescent (Sen BRAF^V600E) melanocytes. Scale bar = 100 μM. i mRNA analysis of p16, p21, IL6, and MMP1. Expression was normalized using Cq values of tubulin. j mRNA expression of Bcl-2, Bcl-xL, and Bcl-w from irradiated melanocytes. Expression was normalized using Cq values of tubulin. k Immunofluorescence and l quantification of Bcl-w positive cells in human nevi. Melan-a was used to confirm the identification of melanocytes or nevus cells. Scale bar = 25 μM. m Viability of non-senescent and BRAF^V600E transduced melanocytes treated with indicated concentrations of ABT-263 for 24 h. n Viability of non-irradiated and irradiated IMR-90 fibroblasts treated with indicated concentrations of ABT-263 for 24 h. Viability of non-irradiated and irradiated melanocytes treated with indicated concentrations of o A-1155463 or p ABT-199 for 24 h. mRNA analysis of NOXA in q BRAF^V600E transduced and r irradiated melanocytes. Expression was normalized using Cq values of tubulin. s Protein expression of NOXA from irradiated melanocytes. Vinculin was used as a housekeeping protein. For all panels, n = 3 independent experiments except d, i, j, and q where n = 4 independent experiments, and s where results are representative of n = 2 independent experiments. Significance was calculated with a two-tailed student’s t test. Error bars = S.E.M. *p < 0.05, **p < 0.01 ***p < 0.001, ns = not significant.