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. 2022 Dec 23;12:22236. doi: 10.1038/s41598-022-26141-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Summarized plot showing the performances of all the methods used to remove unwanted variations in pig faecal samples. For methods utilizing control features (RUVg, RUVs, and RUV-III-NB), we only picked the attempts using combination control taxa (479 taxa) as they demonstrated overall best performances within each method. For every method represented by the stacked bar, each color represents the performance of each method in each specific metric (as previously shown in Fig. 3); for visual uniformity, a taller stacked bar represents overall better performance. For RLE, the value represented was taken from 1-overall canonical correlation of the RLE metrics and known unwanted technical variations; for silhouette (storage), the value was taken from 1- average width of silhouette statistics score for clustering by storage conditions.