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. Author manuscript; available in PMC: 2023 Dec 21.
Published in final edited form as: Neuron. 2022 Oct 5;110(24):4057–4073.e8. doi: 10.1016/j.neuron.2022.09.009

Fig. 2. A brainwide atlas of synapse protein lifetime.

Fig. 2.

(A) PSD95 puncta half-life across 110 mouse brain subregions (Table S1). The 12 main brain areas are color-coded to the right. N=7–9 Psd95HaloTag/HaloTag animals. (B) PSD95 puncta half-life in HPF, with key elements of the circuitry shown. The pyramidal neurons in entorhinal cortex (EC) layer II project to CA1sl, pyramidal neurons in entorhinal cortex layer III project to CA1sm, and stellate neurons in entorhinal cortex layer II project to DG granule neurons, which in turn project to CA3, which project to CA1 (Marks et al., 2020). (C) Brain subregions ranked according to their half-life (± 95% confidence intervals), color-coded as in (A). (D) Fluorescent puncta decay (mean fraction of puncta remaining at day 7 compared with day 0, ± SD) across apical and basal dendrites of the CA1 pyramidal cells. N=3 Psd95HaloTag/HaloTag animals per time point. Reference points for calculating gradients along the apical dendrite are marked by a–d.