KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse Monoclonal anti-parvalbumin (1:500) | Millipore Sigma | Cat#P3088, RRID: AB_477329 |
| Rabbit Polyclonal anti-c-Fos (1:500) | Millipore Sigma | Cat#ABE457, RRID: AB_2631318 |
| Mouse Monoclonal anti-CGRP (1:500) | Abcam | Cat#Ab81887, RRID: AB_1659411 |
| Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 (1:200) | Abcam | Cat#Ab150113,RRID: AB_2576208 |
| Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 546 (1:200) | Thermo Fisher Scientific | Cat#A-10040, RRID: AB_2534016 |
| Oligonucleotides | ||
| Primers for Cre: Forward 5’-CACCCTGTTACGTATAGCCG-3’; Reverse 5’-GAGTCATCCTTAGCGCCGTA-3’ |
Zhong et al., 2018 | N/A |
| Primers for flox site for RARα: Forward 5’-GTGTGTGTGTGTATTCGCGTGC-3’; Reverse 5’-ACAAAGCAAGGCTTGTAGATGC-3’ |
Sarti et al., 2012 | N/A |
| Primers for EYFP: WT Forward: 5’-AAGGGAGCTGCAGTGGAGTA-3’ WT Reverse: 5’-CCGAAAATCTGTGGGAAGTC-3’; Mutant Forward: 5’-ACATGGTCCTGCTGGAGTTC-3’; Mu Reverse: 5’-GGCATTAAAGCAGCGTATCC-3’ |
The Jackson Laboratory | Primer: oIMR9020 oIMR9021 oIMR9102 oIMR9103 |
| Primers for ChR2: WT Forward: 5’-AAGGGA GCTGCAGTGGAGTA-3’; WT Reverse: 5’-CCGAAAATCTGTGGGAAGTC-3’; Mu Forward: 5’-CATTGGTGGCACTGAGATTG-3’; Mu Reverse: 5’-GAACTTCAGGGTCAGCTTGC-3’ |
The Jackson Laboratory | Primer: oIMR9020 oIMR9021 22166 oIMR9402 |
| Primers for FLpO: WT forward: 5’-GGATGCTTGCCGAAGATAAG-3’; Mu forward: 5’-CTGAGCAGCTACATCAACAGG-3’; common reverse: 5’-TGTTTCTCCAGCATTTCCAG-3’ |
The Jackson Laboratory | Primer: 17564 17566 16211 |
| qRT-PCR primer for RARα | ThermoFisher Scientific | Mm00436262_m1 |
| qRT-PCR primer for Actinβ | ThermoFisher Scientific | Mm02619580_g1 |
| qRT-PCR primer for GFAP | ThermoFisher Scientific | Mm01253033_m1 |
| qRT-PCR primer for PV | ThermoFisher Scientific | Mm00443100_m1 |
| qRT-PCR primer for SST | Integrated DNA Technologies | Mm.PT.53a.7678291 |
| qRT-PCR primer for PKCγ | Integrated DNA Technologies | Mm.PT.58.45983184 |
| qPT-PCR primer for Gad1 | Integrated DNA Technologies | Mm.PT.58.10501737 |
| qRT-PCR primer for VGluT2 | Integrated DNA Technologies | Mm.PT.58.10363705 |
| qRT-PCR primer for VGluT3 | Integrated DNA Technologies | Mm.PT.58.17676720 |
| Critical commercial assays | ||
| SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity DNA Polymerase | ThermoFisher Scientific | Cat#12574035 |
| TaqMan Gene Expression Master Mix | Applied Biosystems | Cat#4369016 |
| RNAscope Probe-Mm-Rara | Advanced Cell Diagnostics | Cat#463801 |
| Vectashield Hardset Antifade mounting medium with DPAI | Vector laboratories | Cat#H-1500–10 |
| Bacterial and Virus Strains | ||
| AAV-Frt-Cre-IRES-tdTomato | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Formaldehyde solution | Millipore Sigma | Cat#252549, CAS: 50–00-0 |
| Complete Freund’s Adjuvant | Millipore Sigma | Cat# F5881 |
| Ro41–5253 | Abcam | Cat# Ab141629 CAS: 144092–31-9 |
| Fluorogold | Thermo Fisher Scientific | Cat#NC1363013 |
| Biocytin | Millipore Sigma | Cat#B4261 CAS: 576–19-2 |
| Streptavidin, Alexa Fluor 555 conjugate (1:1000) | Thermo Fisher Scientific | Cat#S21381 |
| CNQX | Tocris Biosciences | Cat#0190 CAS: 115066–14-3 |
| D-APV | Tocris Biosciences | Cat#0106 CAS: 79055–68-8 |
| Bicuculline | Tocris Biosciences | Cat#0131 CAS: 38641–83-7 |
| Strychine | Millipore Sigma | Cat#S0532 CAS: 57–24-9 |
| Experimental Models: Organisms/Strains | ||
| Mouse: PVcre: B6;129P2-Pvalbtm1(cre)Arbr/J | The Jackson Laboratory | JAX: 008069 RRID: IMSR_JAX:008069 |
| Mouse: SSTcre: Ssttm2.1(cre)Zjh/J | The Jackson Laboratory | JAX: 013044 RRID: IMSR_JAX:013044 |
| Mouse: RARαfl/fl: C57BL/6 | Ref (Zhong et al., 2018) | N/A |
| Mouse: Ai3(RCL-EYFP): B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J | The Jackson Laboratory | JAX: 007903 RRID: IMSR_JAX:007903 |
| Mouse: Ai32(RCL-ChR2(H134R)/EYFP): B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J | The Jackson Laboratory | JAX: 012569 RRID: IMSR_JAX:012569 |
| Mouse: Pvalb-T2A-FlpO-D: B6.Cg-Pvalbtm4.1(flpo)Hze/J | The Jackson Laboratory | JAX: 022730 RRID: IMSR_JAX:022730 |
| Software and Algorithms | ||
| Viewer III tracking system | Biobserve | http://www.biobserve.com/behavioralresearch/products/viewer/ |
| ImageJ 1.53 | NIH | https://imagej.nih.gov/ij/ |
| Nikon Elements | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research |
| OlyVIA V3.3 | Olympus | https://www.olympus-lifescience.com/en/support/downloads/ |
| Axon pCLAMP 10 | Molecular Devices | https://support.moleculardevices.com/s/article/Axon-pCLAMP-10-Electrophysiology-Data-Acquisition-Analysis-Software-Download-Page |
| Prism 9 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |