TABLE 2.
Effect of anti-LBP MAb or rmLBP on TNF production by LPS-stimulated PEM cultured with 1% autologous plasmaa
| Conditions | Mean ± SDb for TNF (pg/ml) |
|---|---|
| Plasma-free medium | 30 ± 20 |
| 1% plasma | 1,100 ± 250c |
| 1% plasma + anti-LBP MAb | 20 ± 25 |
| 1% plasma + rmLBP | 700 ± 350c |
PEM (50,000 cells/well) were stimulated for 6 h with 5 ng of LPS/ml in plasma-free medium or in the presence of (i) 1% autologous plasma, (ii) 1% autologous plasma plus 10 μg of anti-LBP MAb (clone M330-19)/ml, or (iii) 1% autologous OF1 plasma plus 100 ng of rmLBP/ml.
Data are from two different experiments run in duplicates.
P < 0.05 by ANOVA.