TABLE 3.

Effect of anti-LBP MAb or rmLBP on TNF production by LPS-stimulated PEM cultured with 10% autologous plasma^a

Conditions	Mean ± SDb for TNF (pg/ml)
1% plasma	1,100 ± 250c
10% plasma	20 ± 20
10% plasma + anti-LBP MAb	20 ± 15
10% plasma + rmLBP	20 ± 25

^aPEM (50,000 cells/well) were stimulated for 6 h with 5 ng of LPS/ml in the presence of (i) 1% (as control) or 10% autologous plasma, (ii) 10% autologous plasma plus 10 μg of anti-LBP MAb (clone M330-19)/ml, or (iii) 10% autologous plasma plus 100 ng rmLBP/ml. 

^bData are from two different experiments run in duplicates. Cells stimulated with LPS in plasma-free medium released less than 30 pg/ml of TNF. 

^cP < 0.05 by ANOVA.