KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-GFP | Chromotek | Cat# PABG1; RRID:AB_2749857 |
| Guinea pig polyclonal anti-SERT | Synaptic Systems | Cat# 340 004; RRID:AB_2620086 |
| Mouse monoclonal anti-ADCY3 | Encor Biotechnology | Cat# MCA-1A12; RRID:AB_2744501 |
| Rabbit anti-PCP4 | Millipore Sigma | Cat# HPA005792; RRID:AB_1855086 |
| Chicken anti-rootletin | Millipore Sigma | Cat# ABN1686; RRID:AB_2893142 |
| Rabbit anti-ADD1 | Abcam | Cat# ab40760; RRID:AB_722627 |
| Rabbit anti-synaptophysin | Cell Signaling | Cat# 36406; RRID:AB_2799098 |
| Rabbit anti-synaptophysin | Thermo Fisher Scientific | Cat# MA5-14532; RRID:AB_10983675 |
| Rabbit anti-Trio GEFD2 | Laboratory of Dr. Susanne Schmidt | N/A |
| Rabbit anti-h4k5ac | Thermo Fisher Scientific | Cat# MA5-32009; RRID:AB_2809303 |
| Mouse anti-panh4ac | Thermo Fisher Scientific | Cat# MA3-066; RRID:AB_2633028 |
| Rabbit anti-H3K27ac | Abcam | Cat# ab177178; RRID:AB_2828007 |
| Alexa Fluor Plus 488 goat anti-rabbit | Thermo Fisher Scientific | Cat# A32731; RRID:AB_2633280 |
| CF488A donkey anti-guinea pig | Biotium | Cat# 20169; RRID:AB_10853115 |
| Alexa Fluor Plus 555 goat anti-rabbit | Thermo Fisher Scientific | Cat# A32732; RRID:AB_2633281 |
| CF555 goat anti-mouse IgG1 | Biotium | Cat# 20247; RRID:AB_10854998 |
| CF633 goat anti-mouse IgG1 | Biotium | Cat# 20250; RRID:AB_10852830 |
| CF633 donkey anti-rabbit | Biotium | Cat# 20215; RRID:AB_10853935 |
| Bacterial and virus strains | ||
| pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] | HHMI Viral Tools | N/A |
| pAAV[flex_on]-CAG-Farnesylated-SNAPtag | HHMI Viral Tools | N/A |
| pAAV-EF1a-DIO-hM3D(Gq)-mCherry | HHMI Viral Tools | N/A |
| pAAV-TRE-HTR6-SNAP-TRIP | HHMI Viral Tools | N/A |
| pAAV-SYN1-Tet3G | HHMI Viral Tools | N/A |
| pAAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE | Addgene | Addgene viral prep # 51509-AAV1 |
| Biological samples | ||
| Hippocampal and raphe neuron culture | In house | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Tn5 transposase | In house | N/A |
| PA-O-Ser | This paper | N/A |
| SB 271046 hydrochloride | Tocris | Cat# 3368; CAS 209481-24-3 |
| SB-258585 hydrochloride | Caymen Chemicals | Cat# 17416; CAS 1216468-02-8 |
| WAY181187 oxalate | Tocris | Cat# 5589; CAS 1883548-85-3 |
| H-89 hydrochloride | Caymen Chemicals | Cat# 10010556; CAS 130964-39-5 |
| Hoechst 33342 | Thermo Fisher Scientific | Cat# 62249; CAS 875756-97-1 |
| Deschloroclozapine | Tocris | Cat# 7193; CAS 1977-07-7 |
| 5-hydroxytrptamine (serotonin, 5-HT) | Alfa Aesar | Cat# B21263-06; CAS 153-98-0 |
| Acetylcholine chloride | Solarbio | Cat# G8320; CAS 60-31-1 |
| Adenosine | Sigma-Aldrich | Cat# A4036; CAS 58-61-7 |
| Adenosine 5’-triphosphate (ATP) | Sigma-Aldrich | Cat# A7699; CAS 34369-07-8 |
| Dopamine hydrochloride | Sigma-Aldrich | Cat# H8502; CAS 62-31-7 |
| γ-Aminobutyric acid (GABA) | Tocris | Cat# 0344; CAS 56-12-2 |
| L-Glutamic acid | Sigma-Aldrich | Cat# V900408; CAS 56-86-0 |
| Glycine | Sigma-Aldrich | Cat# G7403; CAS 56-40-6 |
| Histamine dihydrochloride | Tocris | Cat# 3545; CAS 56-92-8 |
| Melatonin | Sigma-Aldrich | Cat# M5250; CAS 73-31-4 |
| Norepinephrine bitartrate | Tocris | Cat# 5169; CAS 51-40-1 |
| Octopamine hydrochloride | Abcam | Cat# ab120770; CAS 770-05-8 |
| Tyramine | Aladdin | Cat# T105543; CAS 51-67-2 |
| L-Tryptophan | Sigma-Aldrich | Cat# 93659; CAS 73-22-3 |
| Critical commercial assays | ||
| Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG | Thermo Fisher Scientific | Cat# B40922 |
| Experimental models: Cell lines | ||
| hTERT RPE-1 | ATCC | CRL-4000 |
| HEK293A | Laboratory of Dr. Asuka Inoue, https://doi.org/10.1038/ncomms10156 | N/A |
| HEK293A GNAQ/11 KO | Laboratory of Dr. Asuka Inoue, https://doi.org/10.1038/ncomms10156 | N/A |
| HEK293T | ATCC | 1573 |
| Experimental models: Organisms/strains | ||
| Mouse: C56BL/6 | Charles River | Cat# 027; RRID:IMSR_CRL:027 |
| Mouse: C57BL/6J | Jackson Laboratory | Cat# 000664; IMSR_JAX:000664 |
| Mouse: Htr6 KO | This paper | N/A |
| Mouse Htr6-EGFP knock-in | Laboratory of Dr. Séverine Chaumont-Dubel, https://doi.org/10.1073/pnas.1600914113 | N/A |
| Oligonucleotides | ||
| [phos]CTGTCTCTTATACACATCT | Chen et al., 2016 | N/A |
| /ATTO594/TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG | Chen et al., 2016 | N/A |
| /ATTO594/GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG | Chen et al., 2016 | N/A |
| Recombinant DNA | ||
| HTR6-Tango | Kroeze et al., 2015 | Addgene 66414 |
| Tet-On HTR6-RhoA sensor | This paper | N/A |
| Tet-On Arl13b-RhoA sensor | This paper | N/A |
| Tph2-Cre | This paper | N/A |
| pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato | Klapoetke et al., 2014 | Addgene 62723 |
| pAAV[flex_on]-CAG-Farnesylated-SNAPtag | This paper | N/A |
| pAAV-FLEX-tdTomato | Unpublished; Laboratory of Ed Boyden | Addgene 28306 |
| pAAV-EF1a-DIO-hM3D(Gq)-mCherry | Unpublished; Laboratory of Dr. Bryan Roth. | Addgene 50460 |
| pAAV-TRE-HTR6-SNAP-TRIP | This paper | N/A |
| pAAV-SYN1-Tet3G | This paper | N/A |
| Tet-On GRAB-HTR6-cilia:3xGGGGS:Halo tag | This paper | N/A |
| GRAB-HTR6-PM | This paper | N/A |
| Hyperactive piggybac transposase | VectorBuilder | N/A |
| Software and algorithms | ||
| ImageJ/Fiji | Schindelin et al., 2012 | https://imagej.net/software/fiji/ |
| Prism v9.2 | Graphpad Software | https://www.graphpad.com |
| MATLAB 2020b, 2021a | The MathWorks | https://www.mathworks.com/ |
| Python 3.8 (Anaconda) | Anaconda | https://www.anaconda.com/ |
| DABEST | Ho et al., 2019 | https://acclab.github.io/DABEST-python-docs/index.html |
| OrientationJ | Püspöki et al., 2016; Rezakhaniha et al., 2012 | https://github.com/Biomedical-Imaging-Group/OrientationJ |
| VAST Lite | Berger et al., 2018 | https://lichtman.rc.fas.harvard.edu/vast/ |
| 3ds Max 2021 | Autodesk | https://www.autodesk.com/products/3ds-max/overview |
| Mlextend | Raschka, 2018 | http://rasbt.github.io/mlxtend/ |