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IFN-γ and IL-4 production by murine spleen cells (six in each group) stimulated with a mitogen or calpain in vitro. Cytokine production was evaluated in culture supernatants. Data correspond to the mean values from duplicate analyses. Bars: open, calpain-immunized mice; closed, adjuvant-treated controls; hatched, infection controls; dotted, mice immunized with irradiated cercariae. ∗, P < 0.05; ∗∗, P < 0.01.
