FIG. 5.

Kinetics of cytokine production in murine spleen cells stimulated by ConA (A and B) or r-calpain (C and D) in r-calpain-immunized and adjuvant-treated control mice. Cytokines were detected at three different time points. A and C refer to IFN-γ production, and B and D refer to IL-4 production. Cytokine profiles of calpain-immunized mice are shown as circles, and those of adjuvant-treated control mice are shown as open squares. A marked increase in calpain-driven IFN-γ production and a decrease in IL-4 production were observed for the calpain-immunized group compared with the adjuvant-treated control group. ∗, P < 0.05; ∗∗, P < 0.01.