Fig. 4.

NOC1 downregulation in the PG reduces ecdysone production and delays development. (A) Photographs of whole larvae with reduced NOC expression in the PG, driven by the P0206 promoter. Picture represents w¹¹¹⁸ larvae at 5 days AEL and NOC1-RNAi, NOC2-RNAi and NOC3-RNAi larvae at 12 days AEL. (B) Analysis (mean±s.d.) of the size in cells of the FB from control w¹¹¹⁸ or NOC1-RNAi larvae at 5 days and at 12 days AEL. (C) qRT-PCR showing the level of E74b mRNA, target of ecdysone, from whole larvae at the indicated time of development. For B and C, more than 10 animals were used in each experiment from at least two independent experiments. (D–F) Confocal images of the ring gland marked with GFP, using the P0206-GFP driver line, from control w¹¹¹⁸ (D) and from animals with reduced NOC1 at 5 days AEL (E) and 12 days AEL (F). Nuclei are stained with Hoechst. *P<0.05; ****P<0.0001; n.s., not significant (one-way ANOVA with Tukey multi-comparisons test). Images shown are representative of one out of at least three experiments. Scale bars: 1 mm (A); 50 µm (D–F).