TABLE 3.
Resistance to phagocytosis and capsular status of mutants TRF0-6, TRF0-29, and TRF0-30 compared to wild-type S. uberis 0140J grown in CDM containing casein hydrolysate
| Straina | Mean (SD)b
|
|
|---|---|---|
| % Survivalc | μg of capsuled | |
| Wild-type 0140J | 103.02 (15.21) | 145.775 (27.89) |
| TRF0-6 Err | 0.118 (0.06) | 0.325 (0.26) |
| TRF0-6 Ers | 0.440 (0.12) | 0.625 (0.46) |
| TRF0-29 Err | 0.250 (0.15) | 0.750 (0.31) |
| TRF0-29 Ers | 0.135 (0.05) | 0.700 (0.37) |
| Wild-type 0140J | 71.83 (33.67) | 122.35 (18.02)e |
| TRF0-30 Err | 0.16 (0.06) | 1.29 (2.00)e |
| TRF0-30 Ers | 0.22 (0.02) | 1.63 (1.54)e |
Mutant strains were tested before (Err) and after (Ers) pGh plasmid excision.
Unless otherwise indicated, data from triplicate samples from two independently grown broth cultures were pooled (n = 6).
Resistance to phagocytosis is shown as the percent survival of cells relative to cells at time zero.
Capsule status is shown as the amount of hyaluronidase-released N-acetylglucosamine per 1010 bacteria.
Data from quadruplicate samples from two independently grown cultures were pooled (n = 8).