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. Author manuscript; available in PMC: 2022 Dec 24.
Published in final edited form as: Biochemistry. 2022 Jun 23;61(14):1495–1507. doi: 10.1021/acs.biochem.2c00118

Figure 3: The SUN1/2-KASH2 interaction is enhanced by Ca2+ ions and stabilized by oxidizing conditions.

Figure 3:

A and C) Representative confocal fluorescence images of SUPER templates containing the indicated reconstituted EGFP-tagged FL SUN protein constructs after incubation with the indicated TRITC-labeled KASH2 peptides in the presence and absence of 1 mM CaCl2. B and D) Bar graphs depicting the normalized intensity of the indicated TRITC-labeled KASH2 peptides recruited to the SUPER templates containing the indicated EGFP-tagged FL SUN protein constructs in the absence or presence of 1 mM CaCl2. E) Representative confocal fluorescence images of SUPER templates containing the indicated reconstituted EGFP-tagged FL SUN protein constructs following incubation with 10 mM DTT. F) Bar graph depicting the normalized intensity of the indicated TRITC-labeled KASH2 peptides recruited to the SUPER templates containing the indicated EGFP-tagged FL SUN protein constructs following incubation with 10 mM DTT. Scale bars: 5 μm. Error bars represent standard error of the mean. 10–15 beads were analyzed for each condition from n = 3 experiments. ***: p < 0.001 by pairwise Student’s t-test.