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. Author manuscript; available in PMC: 2022 Dec 24.
Published in final edited form as: Biochemistry. 2022 Jun 23;61(14):1495–1507. doi: 10.1021/acs.biochem.2c00118

Figure 4: The CC-containing LDs are important for homo- and hetero-typic SUN protein interactions.

Figure 4:

A) Representative confocal fluorescence images of SUPER templates containing the indicated reconstituted CFE synthesized and GreenLys-labeled FL SUN protein constructs. B) Schematic depicting the workflow for testing the ability of CFE synthesized EGFP-tagged SUN protein LD constructs to be recruited to SUPER templates containing reconstituted CFE synthesized unlabeled FL SUN protein constructs. To control for non-specific protein-protein interactions, the recruitment of soluble CFE synthesized EGFP to SUPER templates containing reconstituted CFE synthesized unlabeled FL SUN protein constructs was tested (bottom left). To control for non-specific interactions between the SUPER template and the CFE synthesized EGFP-tagged SUN protein constructs, the ability of these constructs to be recruited to SUPER templates lacking any reconstituted FL SUN protein constructs was tested (top right). C) Representative confocal fluorescence images of SUPER templates containing the indicated reconstituted CFE synthesized unlabeled FL SUN protein constructs and their ability to recruit the indicated CFE synthesized EGFP-tagged LD SUN protein constructs. Experiments were repeated 3 times. Scale bars: 5 μm.