Figure 1:

Schematic illustration of genetic constructs (top panels) and their corresponding protein variants (bottom panels) used in this work (A-F). A) sCatch-GFP: Split sfCherry_1–10 fused to SpyCatcher and sfGFP without a transmembrane domain. B) sTag-BFP: Split sfCherry₁₁ fused to SpyTag and TagBFP without a transmembrane domain. C) InterCatch: Split sfCherry_1–10 fused to SpyCatcher and single-pass transmembrane domain of transferrin receptor (TfR). D) InterTag: Split sfCherry₁₁ fused to SpyTag and single-pass transmembrane domain of platelet derived growth factor receptor (PDGFR). E) InterCatch-GFP: Split sfCherry_1–10 fused to SpyCatcher, sfGFP and TfR transmembrane domain. F) InterTag-BFP: Split sfCherry₁₁ fused to SpyTag, TagBFP and PDGFR transmembrane domain. (G) SpyTag/SpyCatcher assisted reconstitution of fluorescent sfCherry using transmembrane and soluble versions of InterSpy.