Local cerebral blood flow, measured by laser-speckle imaging, does not increase in response to hindlimb stimulation in the neonatal mouse brain. (A-C) Averaged time courses of the change in oxyhemoglobin (Δ[HbO]), deoxyhemoglobin (Δ[HbR]), tissue hemoglobin (Δ[HbT]), and the percent change in laser-speckle flow (%Δ speckle) in the contralateral hindpaw region of the somatosensory cortex of mice in P7 to P8 (A), P10 to P13 (B), and adult (C) age groups (n = 5, 4, 3, respectively). Source: Adapted, with permission, from Kozberg MG, et al., 2016 (236).