Table 3. Archaea sequence information of rarefied data sets (each primer combination is given in Table 2); all samples were rarefied to 10,000 sequences, except Arc.V46 was rarefied to 4,000 due to high sequence loss for several samples during merging.

	Total archaea reads	Min archaea reads	Max archaea reads	Average archaea reads	Ratio archaea reads (%)	Total ASVs	Archaea ASVs	Ratio archaea ASVs (%)
Prok	293	0	137	29.3	0.3	4,818	5	0.1
Arc.V34	18,393	0	8,200	1,839.3	18.4	1,485	16	1.1
Arc.V46	22,207	0	3,982	2,220.7	55.5	225	22	9.8
Arc.V56	60,706	437	9,979	6,070.6	60.7	732	44	6.1