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Protein profile of native Sapt1p obtained from a culture of C. tropicalis ATCC 750 in BSA medium (lanes 1 and 2) and recombinant Sapt1p (lanes 3 and 4), Sapt2p (lanes 5 and 6), and Sapt3p (lanes 7 and 8) produced in P. pastoris. The gel was stained with Coomassie brilliant blue R-250. Lanes 2, 4, 6, and 8 show proteins deglycosylated after N-glycosidase F treatment. Molecular mass markers (M) are shown in the leftmost lane.
