FIG. 5.

RT-PCR performed with RNA extracted from cells grown for 24 h in YPD (Y) and BSA (B) media. For each reaction, a control without the reverse transcription step (RT −) was done. The sizes of the SAPT1, SAPT2, SAPT3, and SAPT4 PCR products are 1,005, 762, 1,118, and 1,130 bp, respectively. The primers used are described in Table 1.