Table 2.
PCR primers and probes used in the Rapid and Hybrid PCR-Detect assays developed for Karenia brevis
| Primer or probea | Nucleotide sequence (5′ → 3′) | Tm (°C) | Use |
|---|---|---|---|
| Brevis-1-PCR-F | Biotin-TGTTGTCTAAGGTGATAGCTTGC | 62.1 | Forward primer for Rapid and Hybrid |
| Karenia-1-PCR-R | FITC-GAAGCAAATTACCATGTCCCTAG | 62.4 | Reverse primer for Rapid and probe for Hybrid |
| R635-1-PCR-R | Phosphate-GGTCCGTGTTTCAAGACGG | 65.5 | Reverse primer for Hybrid |
Sequences previously published in Goodwin et al., 2005; FITC = fluorescein.