Table 2. The in vitro characteristics of fresh epididymal spermatozoa harvested from WT (n = 24) and BKO (n = 21) mice.
| Sperm characteristics | Mouse genotype | p-value | |
|---|---|---|---|
| WT | BKO | ||
| Concentration (× 106/ml) | 67.6 ± 5.7 | 78.9 ± 5.0 | 0.17 |
| Motility (%) | 45.6 ± 1.8 | 47.1 ± 2.1 | 0.59 |
| Viability (%) | 47.5 ± 2.2 | 47.3 ± 2.7 | 0.95 |
| Plasma membrane integrity (%) | 64.1 ± 1.2 | 60.9 ± 2.1 | 0.18 |
| Acrosome integrity (%) | 39.1 ± 7.1 | 24.7 ± 3.7 | 0.17 |
| MMP (%) | 61.3 ± 2.9 | 59.1 ± 4.7 | 0.69 |
| Oxidative stress | |||
| Live DCF positive sperm (%) | 3.8 ± 1.6 | 10.4 ± 1.7 | 0.01 |
| Mean DCF fluorescence intensity (arb. unit) | 8.4 ± 2.5 | 6.4 ± 1.1 | 1.00 |
Values are mean ± SEM.
n: the total number of mice used in the study (three mice/genotype/replicate).