Fig. 1.

HPLC chromatograms of a low-range and b wide-range ssRNAs obtained using COSMOSIL RNA-RP1-type columns (2.0 mm I. D. x 100 mm; pore sizes are reported in the figure). HPLC analysis was performed with 100 mM TEAA (pH 7.0) using a linear gradient from a 0% to 25% and b 7.5% to 17.5% with acetonitrile over 20 min at a flow rate of 0.2 mL/min and a temperature of 65 °C. UV detection was performed at 260 nm