Fig. 3.

HPLC chromatograms obtained for a low-range and b wide-range ssRNAs using a COSMOSIL RNA-RP1 column (2.0 mm I. D. x 100 mm; particle size, 5 μm). HPLC analysis was performed with 100 mM TEAA-20 mM PB (pH 7.0) using a linear gradient from a 6% to 18.5% and b 10% to 13% with methanol/acetonitrile (50/50, vol/vol) over 20 min at a flow rate of 0.2 mL/min and a temperature of 65 °C. UV detection was performed at 260 nm