Fig. 4.

HPLC chromatograms of a low-range ssRNAs and b low-range dsRNAs obtained using a COSMOSIL RNA-RP1 column (2.0 mm I. D. x 100 mm, particle size; 5 μm) at a flow rate of 0.2 mL/min in the 40 °C–70 °C temperature range. HPLC chromatogram of low-range ssRNA and dsRNA mixtures obtained using a COSMOSIL RNA-RP1 column (2.0 mm I.D. x 100 mm, particle size; 5 μm) at a flow rate of 0.2 mL/min and at a temperature of c 60 °C and d 65 °C. HPLC analysis was performed with 100 mM TEAA (pH 7.0) using a linear gradient from 5% to 20% with acetonitrile over 20 min. UV detection was performed at 260 nm