Fig. 5.

Agarose gel electrophoresis images of a pBR322/MspI and c ΦX174/HaeIII digests. HPLC chromatograms of b pBR322/MspI and d ΦX174/HaeIII digests obtained using a COSMOSIL RNA-RP1 column (4.6 mm I.D. x 100 mm, particle size; 5 μm). HPLC analysis was performed with 100 mM TEAA (pH 7.0) using a linear gradient from b 10% to 17% and d 12% to 17% with acetonitrile over 20 min at a flow rate of 1.0 mL/min and a temperature of 40 °C. UV detection was performed at 260 nm