Fig. 6.

a Scheme and electrophoresis image of the synthesized 3.0 knt and 4.8 knt pQBI T7-GFP RNA. HPLC chromatograms of b 3.0 knt and c 4.8 knt RNA obtained using a COSMOSIL RNA-RP1 (2.0 mm I.D. x 100 mm; particle size, 5 µm) column. HPLC analysis was performed with 100 mM TEAA-20 mM PB (pH 7.0) using a linear gradient from 11% to 13% with methanol/acetonitrile (50/50, vol/vol) over 20 min at a flow rate of 0.2 mL/min and a temperature of 60 °C. UV detection was performed at 260 nm