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. 2022 Dec 24;13(12):1070. doi: 10.1038/s41419-022-05515-z

Fig. 6. UHRF1 liposomal siRNA could block PF progression in mouse models.

Fig. 6

A Schematic diagram of UHRF1 siRNA liposome or scramble liposome together with silica-treated mouse experiment. B Representative HE, Sirius red, UHRF1 IHC, and Masson staining of lung tissues from each group of C57BL/6 mice sacrificed on day 28 (scale bars, 100 µm). C Representative immunofluorescence staining of α-SMA and Collagen I from mouse lung tissues (scale bars, 100 µm). D Ashcroft score of mice from each group. E The labile iron concentration of lung tissues was assessed using an Iron Colorimetric Assay Kit. F The levels of hydroxyproline content were determined at 550 nm and expressed as micrograms per mg of lung tissues, determined by the hydroxyproline content assay kit. G The protein levels of Fibronectin, Collagen I, α-SMA in lung tissues, and UHRF1, FSP1, GPX4 in primary AEC2 cells from each group were detected by western blot and qualified (means ± SD, n = 3). *P < 0.05 versus control group, **P < 0.01 versus control group, #P < 0.01 versus silica+scramble liposomes group.