Table 5.
Constituents of HE from run V1 as determined by LC‐DAD‐MS n analysis
| Constituent | Retention time (min) | [M + H]+ | MS2 | MS3 | Concentration (mg g−1) | Reference |
|---|---|---|---|---|---|---|
| Cannabinoids (neutral forms) | ||||||
| Hydroxy‐ tetrahydrocannabinol | 24.8 | 331 |
313 273 221 205 193 181 133 |
271 243 231 193 |
1.90 ± 0.05 | 36 |
| Unidentified cannabinoid | 25.6 | 315 |
259 233 193 135 123 |
231 217 189 161 |
3.04 ± 0.07 | |
| Cannabidivarin a | 26.1 | 287 |
231 205 193 165 153 135 |
3.37 ± 0.08 | 37 | |
| Hydroxy‐cannabidiol | 27.8 | 331 |
313 193 |
271 257 243 231 193 |
3.85±0.06 | 36 |
| Cannabidiol a | 28.3 | 315 |
259 233 193 135 123 |
231 217 189 161 |
160.45 ± 0.11 | |
| Cannabinol a | 29.7 | 311 |
293 241 223 |
223 208 195 |
14.95 ± 0.09 | 37 |
| Tetrahydrocannabinol isomer 1 | 30.3 | 315 |
259 233 193 135 123 |
231 217 189 161 |
2.74±0.04 | 37 |
| Tetrahydrocannabinol isomer 2 | 30.7 | 315 |
259 233 193 135 123 |
231 217 189 161 |
9.70 ± 0.08 | 37 |
| Cannabicyclol | 31.3 | 315 |
259 233 193 135 123 |
231 217 189 161 |
0.09 ± 0.01 | 37 |
| Total | 200.10 | |||||
| Unidentified | 30.9 | 593 | 533 |
461 433 477 417 |
9.78 ± 0.08 | |
| Cannabinoids (acid forms) | ||||||
| Hydroxy‐cannabidivarinic acid derivative | 22.3 | 347 | 329 |
311 205 |
2.38 ± 0.03 | |
| Cannabidivarinic acid | 23.5 | 329 | 311 |
268 173 |
3.04 ± 0.03 | 31 |
| Hydroxy‐cannabidivarinic acid derivative | 24.1 | 345 |
327 309 |
309 285 |
1.36 ± 0.02 | |
| Hydroxy‐cannabidivarinic acid derivative | 24.6 | 345 |
327 285 271 219 191 |
0.85 ± 0.01 | ||
| Cannabidiolic acid a | 27.7 | 357 | 339 |
324 295 271 227 |
14.35 ± 0.09 | |
| Cannabidiolic acid derivative | 29.0 | 525 | 339 |
295 271 227 |
4.28 ± 0.06 | |
| Total | 26.25 | |||||
| Geranyl flavones | ||||||
| Cannflavin C a | 25.0 | 435 | — | — | 0.01 ± 0.01 | 38 |
| Cannflavin A a | 26.9 | 435 |
420 351 |
336 323 309 |
0.26 ± 0.01 | 38 |
| Total | 0.27 | |||||
a
Indicates identification with reference standards.