Fig. 3.

Secretion of mucin from primary human conjunctival goblet cell (GC) cultures assessed through immunohistochemical stainings. GCs were incubated for 30 minutes with culture medium as a negative control, polyquarternium‐1 (PQ)‐preserved Travatan or benzalkonium chloride (BAK)‐preserved Travoprost Stada. Travoprost Stada represents the BAK‐preserved generics. The nucleus was blue with DAPI (4′,6‐diamidino‐2‐phenylindole), the cytoskeleton green with anti‐cytokeratin‐7 and the mucin red with MUC5AC. The three stainings were merged. Dispersion of mucin to the cytoplasma indicates a secretagogue effect for both BAK‐ and PQ‐preserved travoprost. Stainings were performed on cultures from three individual donors. Scale bar = 100 μm. [Colour figure can be viewed at wileyonlinelibrary.com]