TABLE 3.

Effect of LPS pretreatment on blood leukocytes during serovar Typhimurium infection^a

Time (h)	Pretreatment	Total leukocytes (106/ml)	% PMN	% Lymphocytes	% Monocytes
1	Control	2.3 ± 1.6	18 ± 8	70 ± 11	9 ± 5
	LPS i.p.	5.2 ± 1.9b	76 ± 6b	14 ± 6c	10 ± 2
	LPS i.v.	2.9 ± 1.0	51 ± 8b	35 ± 6c	14 ± 4
48	Control	1.2 ± 1.0	51 ± 14	34 ± 15	12 ± 8
	LPS i.p.	4.9 ± 2.4c	61 ± 11	27 ± 7	10 ± 7
	LPS i.v.	3.3 ± 1.3c	68 ± 3	20 ± 4	12 ± 2

^aMice were treated i.p. or i.v. with serovar Abortus equi LPS (1 mg/kg) three times at 24-h intervals. Twenty-four hours after the last LPS injection, LPS-tolerant mice and saline-pretreated controls were infected with serovar Typhimurium (10⁷ bacteria/kg i.p.). Total and differential counts of blood leukocytes were performed 1 and 48 h after infection. Data are expressed as means ± SD (n = 9 for saline controls and n = 6 for the LPS groups). 

^bSignificantly different from saline control (P < 0.05). 

^cSignificantly different from control (P < 0.01).